ABSTRACT
The oligonucleotide primer having the 5’\|GCCGGTTATGGC AGCACGCTGACC\|3’ was synthesized,which encodes a main repetition motif of protein produced by the inaZ gene.PCR of the primer in the DNA of INA - and INA + bacteria and fungi was done.All of the DNA of INA - and INA + bacteria and fungi expressed bands,the results show that the primer did not work for distinguishing the INA - and INA + organisms and for identification of the ice nucleation activity of the INA organisms by the size and brightness of the PCR products.
ABSTRACT
The effect of 5 store methods at 3 different temperature on ice nucleation activity of INA bacteria were evaluated. The results showed that some store methods could decrease the ice nucleation activity. The effect increased as the temperature of increased. The effect on survival and on activity is different. Different strains had different susceptibility and adaptability. It was found that the freeze-drying method storing at -20℃ and freezing in sterile distilled water storing at -20℃ could be used for most INA bacteria. Freezing in 10% glycerin could not be used for the store of INA bacteria.